Journal: STAR Protocols

Article Title: Protocol for whole-cell patch-clamp recording and post hoc identification of hippocampal CA2 pyramidal neurons in adult mouse brain slices

doi: 10.1016/j.xpro.2026.104470

Figure Lengend Snippet: Passive properties of CA2 pyramidal neurons and miniature IPSCs (A) Capacitive and leakage currents in a CA2 pyramidal neuron in response to a 5 mV voltage step recorded at a holding potential of Vh=−70 mV before the current trace in A. The series resistance Rs is 8.1 MΩ. Capacitive transients are fitted with the sum of two exponentials giving time constants of 30 and 355 pF. Read out value from clampEx was Cm=210 pF. Current trace is an average of 9 raw traces. (B) Capacitive and leakage currents acquired after the current trace in C to show the absence of gross change in the passive properties of the cell and recording conditions. Rs is 8.8 MΩ (less than 9% change in comparison to condition in A). Average current trace from 11 raw traces. Current traces in A and B are recorded in the presence of 20 μM D-AP5, 10 μM CNQX, and 1 μM TTX.

Article Snippet: D-AP5 , Hello Bio , Cat#HB0225.

Techniques: Comparison

Journal: STAR Protocols

Article Title: Protocol for whole-cell patch-clamp recording and post hoc identification of hippocampal CA2 pyramidal neurons in adult mouse brain slices

doi: 10.1016/j.xpro.2026.104470

Figure Lengend Snippet: Excitatory and inhibitory spontaneous synaptic currents in CA2 pyramidal neurons (A) Representative recording of mIPSCs at a holding potential of Vh=−70 mV. 20 μM D-AP5, 10 μM CNQX, and 1 μM TTX are added to external solution to block excitatory synaptic activity and spontaneous AP firing. Green dashed vertical lines indicate the sections of current selected for expanded time view in B. Current trace sections are labeled #1 to #5. (B) Selection of five current trace sections extracted from current trace in (C). (C) Miniature currents selected using Clampfit with template matching selection method. Superimposition of 23 individual current traces in black with one in red. (D) Current traces from (C) at expanded time scale. (E) Original trace of sEPSCs recorded at a holding potential of Vh=−70 mV. 50 μM picrotoxin was added to external solution to block inhibitory synaptic activity. Blue dashed vertical lines indicate the sections of current selected for expanded time view in F. (F) Five current trace segments (blue label #1 to #5) extracted from the whole current trace in (E) at expanded time scale. (G) Spontaneous excitatory postsynaptic currents selected using Clampfit with template matching selection method. Superimposition of 12 individual current traces in black with one in red. (H) Current traces from (G) at expanded time scale.

Article Snippet: D-AP5 , Hello Bio , Cat#HB0225.

Techniques: Blocking Assay, Activity Assay, Labeling, Selection

Journal: Endocrinology

Article Title: Robust serotonin activation of the kisspeptin GnRH pulse generator in male and female mice

doi: 10.1210/endocr/bqag034

Figure Lengend Snippet: Effects of serotonin and 5-HT receptor compounds on ARN KISS neuron firing in acute brain slices from diestrous female mice. (A) Robust excitatory effect of 90 seconds puff of 40 µM serotonin on firing rate of a middle ARN kisspeptin neuron. (B) Inhibitory effect of 90 seconds puff of 60 µM serotonin on firing rate of a caudal ARN kisspeptin neuron initially stimulated to fire by a 200 nM puff of NKB. (C) Rostral ARN kisspeptin neuron not responding to 60 µM puffs of serotonin but later activated by NKB. (D) Summary of percentage of rostral (rARN), middle (mARN), and caudal (cARN) kisspeptin neurons excited or inhibited by serotonin. (E) Caudal ARN kisspeptin neuron activated by serotonin (40 µM) in the absence and presence of methiothepin (100 µM). (F) Middle ARN kisspeptin neuron in which the excitatory effect of serotonin (40 µM) is blocked by methiothepin (100 µM). (G) Middle ARN kisspeptin neuron activated by serotonin (40 µM) in the absence and presence of SB228357 (100 µM). (H) Caudal ARN kisspeptin neuron in which zacopride (2 µM) facilitates serotonin excitation. (I) Whole-cell recording from an ARN KISS neuron in the continuous presence of TTX, CNQX, DAP5, and bicuculline, showing depolarization during bath application of 5-HT (30 µM). (J) Summary of mean membrane potential measured predrug, during 5-HT application, and following wash ( P < .001; n = 4 animals).

Article Snippet: Stock solutions of GABAzine (SR95531, 5 mM, Tocris, UK), CNQX (10 mM, Tocris, UK), and DAP5, (50 mM, Tocris, UK) were prepared with Milli-Q water or NaOH for DAP5.

Techniques: Membrane